Microflora, enzyme activity and humus status indices of agro dark humus bleached soil under the conditions of phytomeliorative experiment
Author(s):
Shchapova L.N., Purtova L.N., Kiseleva I.V.
DOI: 10.31433/2618-9593-2019-22-3-38-44
PDF (1234 К)
Abstract:
Changes in the number and composition of microfl ora of agro dark humus bleached soil in the cultivation of various phytomeliorants in the conditions of short-term fi eld experiments are established (1 и 3 years) are established. On the variants with the sowing of phytomeliorants, there is a pronounced tendency for an increase in the content and reserves of humus as compared with the control. Changes in the indicators of the humus state and microbiological activity of the soils were traced. Under the conditions of one-year experience, the highest number of ammonifying microorganisms showed the variant with buckwheat. This led to increased activity of polyphenol oxidases and more intensive accumulation of humus. The mineralization ratio decreased markedly. The mobility of humic acids increased as compared with the control; the humus system was in an unstable state. In the variant with sowing of clover, the mineralization processes were most pronounced due to the decrease in the share of ammonifi сators, but due to the low activity of peroxidase, the humus accumulation coeffi cient had average values. The composition of humus dominated by the fraction associated with calcium. Humus on all variants, under the conditions of one-year experience, had a fulvate-humate composition. In three-years phytomeliorative experience, a high intensity of mineralization processes was observed in the soil under crops of the timothy grass + clover. The type of humus formation changed to humate-fulvate.The mobility of humic acids increased. The activity enzymes and coeffi cient of humus accumulation in this variant was low. The most favorable ratio of the processes of mineralization and accumulation of humus was established on the variant under the alfalfa in the three-years phytomeliorative experience, which was characterized by higher humus reserves and the humate type of humus formation, high polyphenol oxidase activity and humus accumulation coeffi cient. High polyphenol oxidase activity and humus accumulation coeffi cient are characteristic of the process. The proportion of the 2nd fraction of humic acids increased to high values, indicating a more stable state of the soil humus system.
Keywords:
phytomeliorants, microfl ora, biogenicity, peroxidase, polyphenolokidase, humus content and reserves, humic acids, catalase activity
References:
1. Галстян А.Ш. Об устойчивости ферментов почв // Почвоведение. 1982. № 4. С. 108–110.
2. Дырин В.А. Активность пероксидазы и полифенодоксидазы в торфе целинного и рекультивируемого участков в болотных экосистемах низинного типа // Вестник Томского государственного педагогического университета. 2015. № 2 (155). С. 164–165.
3. Казеев К.Ш., Колесников С.И., Вальков В.Ф. Биологическая диагностика и индикация почв: методология и методы исследований. Ростов-на-Дону: Изд-во ЦВВР, 2004. 350 с.
4. Классификация и диагностика почв России. Смоленск: Ойкумена, 2004. 342 с.
5. Методы почвенной микробиологии и биохимии / под общ. ред. Д.Г. Звягинцева. М.: Изд-во МГУ, 1991. 303 с.
6. Ознобихин В.И., Синельников Э.П. Характеристика основных свойств почв Приморья и пути их рационального использования. Уссурийск: ПСХИ, 1985. 72 с.
7. Орлов Д.С., Гришина Л.А. Практикум по химии гумуса: учеб. пособие. М.: Изд-во Моск. ун-та, 1981. 272 с.
8. Раськова Н.В. Активность и свойства пероксидазы и полифенолокскдазы в дерново-подзолистых почвах под лесными биоценозами // Почвоведение. 1995. № 11. С. 1363–1368.
9. Рыбачук Н.А., Ознобихин В.И. Разработка методики картографической оценки трансформации и динамики агрохимических показателей пахотных почв // Труды Дальневост отд. Докучаевского о-ва РАН. Владивосток: ДВО РАН, 2005. Т. 2. С. 12–18.
10. Хазиев Ф.Х. Методы почвенной энзимологии. М.: Наука, 2005. 252 с.
11. Чундерова А.И. Активность полифенолоксидазы и пероксидазы в дерново-подзолистых почвах // Почвоведение. 1970. № 7. С. 22–26.
12. Ndour Ndèye Yacine Badiane, Chotte J.L, Pate E, Masse D, Rouland C. Use of soil enzyme activities to monitor soil quality in natural and improved fallows in semi-arid tropical regions // Applied Soil Ecology. 2001. Vol. 18, Is. 3. P. 229–238.
13. Orlov D.S., Biryukova O.N., Rozanova M.S. Revised system of the humus status parameters of soils and their genetic horizons // Eurasian Soil Science. 2004. Vol. 37, N 8. P. 798–805.
14. Suyundukov Yа.T., Mirkin B.M., Abdullin M.R., Sal'manova E.F., Hasanova G.R. The effect of phytoamelioration on the fertility of chernozems in the Trans-Ural part of Bashkiria // Eurasian Soil Science. 2007. Vol. 40, N 10. P. 1087–1094.
